Cholestest N HDL

Cholestest N HDL is a liquid reagent for the measurement of HDL-cholesterol by the direct method, using differences in specificities of detergent to lipoprotein. The reagent does not contain polyanions or magnesium salts, allowing the accurate measurement of samples with high levels of globulin or TG. The reagent will not form insoluble precipitates upon the addition of an alkaline washing solution, therefore there will not be any burden on the instrument.

  • Accurate measurement of samples with high globulin or triglyceride levels
  • Does not contain flocculants or divalent metal ions
  • Good correlation with DCM method
  • *This package insert follows the Pharmaceuticals, Medical devices and Other Therapeutic Products Act of Japan.
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Description

*Read carefully the package insert before use.

Performance Data

Reference values measured with the Hitachi 7180

Reproducibility

(mg/dL)

  Sample 1 Sample 2 Sample 3
n 20 20 20
Mean 38.72 90.78 53.77
S.D. 0.38 0.54 0.47
C.V. 0.98 0.59 0.87
Max. 39.6 91.9 54.5
Min. 38.1 89.8 53.0
Range 1.5 2.1 1.5

Linearity

Linearity

Correlation

Correlation between SEKISUI and DCM method(in-house data)

Explanation

The HDL-cholesterol direct assay reagent “Cholestest N HDL” manufactured and marketed by SEKISUI MEDICAL CO., LTD., is designed to comply with the DCM method (dextran sulfate magnesium precipitation separation method).

Correlation between SEKISUI and Ultracentrifuge method*1

Explanation

It has been reported that the correlation with the ultracentrifugation method was not observed in serum samples (TG 259 – 1497 mg/dL) of patients with hypertriglyceridemia.

<References * 1> Yoshikazu Ono, et al., Clinical Science 2002: 31: 44 -50.

Interference

Unconjugated Bilirubin

Unconjugated Bilirubin

(mg/dL)

Added Concentration
(mg/dL)
Measured Value %
0.0 49.00 100.0%
4.0 49.80 101.6%
8.0 50.10 102.2%
12.0 50.60 103.3%
16.0 49.90 101.8%
20.0 50.75 103.6%

Conjugated Bilirubin

Conjugated Bilirubin

(mg/dL)

Added Concentration
(mg/dL)
Measured Value %
0.0 49.30 100.0%
4.0 49.40 100.2%
8.0 49.90 101.2%
12.0 49.70 100.8%
16.0 49.75 100.9%
20.0 50.00 101.4%

Hemoglobin

Hemoglobin

(mg/dL)

Added Concentration
(mg/dL)
Measured Value %
0 49.65 100.0%
100 50.00 100.7%
200 49.85 100.4%
300 49.90 100.5%
400 50.25 101.2%
500 50.50 101.7%

Ascorbic acid

Ascorbic acid

(mg/dL)

Added Concentration
(mg/dL)
Measured Value %
0 50.55 100.0%
10 50.35 99.6%
20 50.20 99.3%
30 50.15 99.2%
40 49.90 98.7%
50 50.20 99.3%

Chyle

Chyle

(mg/dL)

Added Concentration
(FTU)
Measured Value %
0 49.75 100.0%
600 49.45 99.4%
1200 49.40 99.3%
1800 49.55 99.6%
2400 48.90 98.3%
3000 48.95 98.4%

Measurement Range(Hitachi 7170 Automated Analyzer)

2-150mg/dL

Cholestest N HDL

Additional information

Cholestest N HDL|Enzyme Solution 1

2×20mL|2×25mL|Storage: 2-10℃

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