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CV is approximately 5% at 1 ng/mL and approximately 3% at around 2 ng/mL, showing good precision.
Reactivities of free and bound PSA
Using free PSA and bound PSA of the same concentration, samples prepared by changing the mixing ratio of free and bound PSA were measured. According to the results, results did not change in the measurement region, from 0 to 100 percent of free PSA. This shows that free and bound forms are measured equally (equimolar response).
Detection limit and effective sensitivity
The detection limit was found to be 0.5 ng/mL using the method of Mean ±2.6SD at zero concentration, and 0.4 ng/mL using an effective sensitivity 20 percent of CV for between-run precision. Both results show that the detection limit is approximately 0.5 ng/mL.
Linearity was observed at a concentration of up to 100 ng/mL.
There was high correlation between our method and the RIA method which is accepted as the standard method.